To set the stage I will introduce the phenotypic screening and the relevance of this approach to drug discovery. The talk will highlight a number of image analysis techniques that play an increasingly important role in phenotypic screening. In particular it will review algorithms for cell tracking and cell cycle estimation as well as image analysis based approaches for tissue mapping. Apart from discussing the image analysis algorithms the presentation will also outline what work will be necessary to integrated the high-content information in the overall workflow. The ongoing work at the newly established Target Discovery Institute (TDI) at the University of Oxford will also be presented. The overall goal and the research objectives of the different groups at TDI will be discussed.